Lewin's genes XII / Jocelyn E. Krebs, Elliott S. Goldstein, Stephen T. Kilpatrick.

4.5 Eukaryotic Protein-Coding Genes Can Be Identified by the Conservation of Exons and of Genome Organization.

Includes bibliographical references and index.

Cover Page; Title Page; Copyright Page; Dedication; Brief Contents; Contents; Preface; About the Authors; PART I Genes and Chromosomes; Chapter 1 Genes Are DNA and Encode RNAs and Polypeptides; 1.1 Introduction; 1.2 DNA Is the Genetic Material of Bacteria and Viruses; 1.3 DNA Is the Genetic Material of Eukaryotic Cells; 1.4 Polynucleotide Chains Have Nitrogenous Bases Linked to a Sugar-Phosphate Backbone; 1.5 Supercoiling Affects the Structure of DNA; 1.6 DNA Is a Double Helix; 1.7 DNA Replication Is Semiconservative; 1.8 Polymerases Act on Separated DNA Strands at the Replication Fork.

1.9 Genetic Information Can Be Provided by DNA or RNA1.10 Nucleic Acids Hybridize by Base Pairing; 1.11 Mutations Change the Sequence of DNA; 1.12 Mutations Can Affect Single Base Pairs or Longer Sequences; 1.13 The Effects of Mutations Can Be Reversed; 1.14 Mutations Are Concentrated at Hotspots; 1.15 Many Hotspots Result from Modified Bases; 1.16 Some Hereditary Agents Are Extremely Small; 1.17 Most Genes Encode Polypeptides; 1.18 Mutations in the Same Gene Cannot Complement; 1.19 Mutations May Cause Loss of Function or Gain of Function; 1.20 A Locus Can Have Many Different Mutant Alleles.

1.21 A Locus Can Have More Than One Wild-Type Allele1.22 Recombination Occurs by Physical Exchange of DNA; 1.23 The Genetic Code Is Triplet; 1.24 Every Coding Sequence Has Three Possible Reading Frames; 1.25 Bacterial Genes Are Colinear with Their Products; 1.26 Several Processes Are Required to Express the Product of a Gene; 1.27 Proteins Are trans-Acting but Sites on DNA Are cis-Acting; Chapter 2 Methods in Molecular Biology and Genetic Engineering; 2.1 Introduction; 2.2 Nucleases; 2.3 Cloning; 2.4 Cloning Vectors Can Be Specialized for Different Purposes; 2.5 Nucleic Acid Detection.

2.6 DNA Separation Techniques2.7 DNA Sequencing; 2.8 PCR and RT-PCR; 2.9 Blotting Methods; 2.10 DNA Microarrays; 2.11 Chromatin Immunoprecipitation; 2.12 Gene Knockouts, Transgenics, and Genome Editing; Chapter 3 The Interrupted Gene; 3.1 Introduction; 3.2 An Interrupted Gene Has Exons and Introns; 3.3 Exon and Intron Base Compositions Differ; 3.4 Organization of Interrupted Genes Can Be Conserved; 3.5 Exon Sequences Under Negative Selection Are Conserved but Introns Vary; 3.6 Exon Sequences Under Positive Selection Vary but Introns Are Conserved.

3.7 Genes Show a Wide Distribution of Sizes Due Primarily to Intron Size and Number Variation3.8 Some DNA Sequences Encode More Than One Polypeptide; 3.9 Some Exons Correspond to Protein Functional Domains; 3.10 Members of a Gene Family Have a Common Organization; 3.11 There Are Many Forms of Information in DNA; Chapter 4 The Content of the Genome; 4.1 Introduction; 4.2 Genome Mapping Reveals That Individual Genomes Show Extensive Variation; 4.3 SNPs Can Be Associated with Genetic Disorders; 4.4 Eukaryotic Genomes Contain Nonrepetitive and Repetitive DNA Sequences.

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